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    Biosess标记抗体高分文献引用精选


    Ashok Kumar Jayavelu. et al. Splicing factor YBX1 mediates persistence of JAK2 -mutated neoplasms. Nature. 2020 Nov;588(7836):157-163.

    Nature2020 Pubmed ID:33239784

    产品编号:Anti-JAK2/Cy3 (bs-0908R-Cy3)

    IF: 42.778 Application: IF Species: Mouse


    Zhai, Yihui. et al. T lymphocyte membrane-decorated epigenetic nanoinducer of interferons for cancer immunotherapy. Nat Nanotechnol. 2021 Sep;:1-10.

    Nature Nanotechnology2021 Pubmed ID:34580467

    产品编号:Anti-LAMP2/FITC (bs-2379R-FITC)

    IF: 39.213 Application: IF Species: Mouse


    Alivernini S et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020 Aug;26(8):1295-1306.

    Nature Medicine2020 Pubmed ID:32601335

    产品编号:Anti-MERTK/Cy3 (bs-0548R-Cy3)

    IF: 36.13 Application: IF Species: Human


    Emeric Limagne. et al. MEK inhibition overcomes chemoimmunotherapy resistance by inducing CXCL10 in cancer cells. Cancer Cell. 2022 Jan.

    Cancer Cell2022 Pubmed ID: 35051357

    产品编号:Anti-C-Myc/AF594 (bs-4963R-AF594)

    IF: 31.743 Application: IF Species: Mouse


    Dumont, Nicolas A., et al. "Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division." Nature Medicine (2015).

    Nature Medicine2015 Pubmed ID:26569381

    产品编号:Anti-phospho-MARK2(Thr596)/AF647 (bs-5742R-AF647)

    IF: 30.357 Application: IF Species: Mouse


    Lu, Yiwen. et al. Eosinophil extracellular traps drive asthma progression through neuro-immune signals. Nat Cell Biol. 2021 Oct;:1-13.

    Nature Cell Biology2021 Pubmed ID:34616019

    产品编号:Anti-EAR1/AF555 (bs-1754R-AF555)

    IF: 28.824 Application: IF Species: Human


    Homan KA et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro. Nat Methods. 2019 Mar;16(3):255-262.

    Nat Methods2019 Pubmed ID:30742039

    产品编号:Anti-VEGFR2/AF555 (bs-10412R-AF555)

    IF: 28.467 Application: FCM Species: Human


    Cai, Baohuan. et al. IRAK1-regulated IFN-γ signaling induces MDSC to facilitate immune evasion in FGFR1-driven hematological malignancies. Mol Cancer. 2021 Dec;20(1):1-20.

    Molecular Cancer2021 Pubmed ID:34906138

    产品编号:Anti-NKG2D/AF555 (bs-0938R-AF555)

    IF: 27.401 Application: FCM Species: Mouse


    Cheng Gao. et al. Surface-engineered chlorella alleviated hypoxic tumor microenvironment for enhanced chemotherapy and immunotherapy of first-line drugs. MATER TODAY. 2022 Jul.

    Materials Today2022 Pubmed ID:10.1016/j.mattod.2022.06.024

    产品编号:Anti-HIF-1 Alpha/AF555 (bs-0737R-AF555)

    IF: 26.943 Application: IF Species: Mouse


    Eszter Á. Tóth. et al. Formation of a protein corona on the surface of extracellular vesicles in blood plasma. J Extracell Vesicles. 2021 Sep;10(11):e12140.

    Journal of Extracellular Vesicles2021 Pubmed ID: 34520123

    产品编号:Anti-Fibrinogen alpha chain/FITC (bs-7548R-FITC)

    IF: 25.841 Application: Immune electron microscopy Species: Human


    Li, Fengyin, et al. "Prostaglandin E1 and Its Analog Misoprostol Inhibit Human CML Stem Cell Self-Renewal via EP4 Receptor Activation and Repression of AP-1." Cell Stem Cell 21 (2017): 1-15.

    Cell Stem Cell2017 Pubmed ID:28844837

    产品编号:Anti-EP3/PTGER3/AF647 (bs-1876R-AF647)

    Anti-Prostaglandin E Receptor EP1/AF647 (bs-6316R-AF647)

    IF: 23.394 Application: FCM Species: Mouse


    Yamamizu, Kohei, et al. "Protein Kinase A Determines Timing of Early Differentiation through Epigenetic Regulation with G9a." Cell stem cell 10.6 (2012): 759-770.

    Cell Stem Cell》2012 Pubmed ID: 22704517

    产品编号:Anti-FOXA2/PE (bs-2358R-PE)

    Anti-Nestin/PE (bs-0008R-PE)

    IF: 22.634 Application: FCM Species: Mouse


    Ni L et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity 2020 06 16;526(6).

    Immunity》2020 Pubmed ID:32413330

    产品编号:Goat Anti-Human IgM / HRP (bs-0345G-HRP)

    IF: 22.553Application: ELISA Species: Human


    Shogo Kumagaiet al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells. Immunity. 2020 Jul 14;53(1):187-203.e8.

    Immunity》2020 Pubmed ID:32640259

    产品编号:Anti-PPAR alpha/AF647 (bs-3614R-AF647)

    Anti-PPAR Gamma/PE (bs-4590R-PE)

    IF: 22.553 Application: FCM Species: Human


    Lin Yao. et al. Nasal Spray of Neutralizing Monoclonal Antibody 35B5 Confers Potential Prophylaxis Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants of Concern (VOCs): A Small-scale Clinical Trial. CLIN INFECT DIS. 2022 Jun.

    CLINICAL INFECTIOUS DISEASES》2022 Pubmed ID:35666466

    产品编号:Goat Anti-Human IgG H&L / HRP (bs-0297G-HRP)

    IF: 20.999 Application: ELISA Species: Human


    Miller A et al. ""Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments."" Cell Metabolism(2017)."

    Cell Metabolism》2017 Pubmed ID: 28889950

    产品编号:Anti-FAPA/AF647 (bs-5758R-AF647)

    Anti-CD31/AF488 (bs-0468R-AF488)

    IF: 20.565 Application: IF Species: Human


    van Ree JH et al. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. (2016) Nat.Cell.Bio. 18:814-21.

    Nat Cell Biol》2016 Pubmed ID:27240320

    产品编号:Anti-SLC33A1/FITC (bs-0699R-FITC)

    IF: 20.06 Application: IF Species: Mouse





    标记抗体服务



    服务宗旨:高品质的标记原料采购,规范的标记操作流程,严格的标记产品质控,合理的市场价格定位。
    服务目标:以国际高标准的标记服务水平,提供高品质的标记抗体产品,助力科研人员轻松科研。



    一、服务内容:

    本服务项目主要为接收客户的抗体、蛋白、多肽、小分子化合物进行常规标记物标记。

    1. 可接收的待标记样品包含抗体IgG、大分子蛋白、小分子多肽、小分子化合物等。

    2. 可提供标记服务的标记物有:

    A. 酶标记物:碱性磷酸酶(AP/ALP),辣根过氧化物酶(HRP);

    B. 生物素(Biotin);

    C. 常规荧光素:FITC,Rhodamine B(RBITC);

    D. Cy系列荧光素:Cy3,Cy5,Cy5.5, Cy7;

    E. 荧光蛋白:藻红蛋白(PE),别藻蓝蛋白(APC),PerCP;

    F. 荧光双标记:PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7;

    G. 胶体金标记Co-Au;

    二、服务流程:

    1.  客户提交委托标记请求并明确标记要求,与公司商定具体的实验项目、数量和费用,双方签订《委托标记制备合同》。

    2.  客户提供待标记样品,我公司按照合同要求安排标记服务,并在规定时间内完成标记委托。

    3.  标记完成后,我公司按照客户要求分装,免费低温邮寄给客户,同时提供标记报告。

    三、样品要求:

    1.  待标记样品需保证90%以上的纯度,样品中不含有干扰标记物结合的成分,如有特殊成分,请提前标注说明。

    2.  待标记样品最小标记量为1mg,最好是干粉,如果是液体,浓度应大于1mg/ml。

    3.  抗体IgG样品中应不含BSA、叠氮钠、甘氨酸等成分。

    4.  大分子蛋白应提供分子量、等电点、缓冲液成分及pH等信息;小分子多肽需提供氨基酸序列;小分子化合物还需提供分子结构式。

    5.  请提前说明任何特殊情况。

    四、收费标准:

    1.  抗体和大分子蛋白标记收费标准:

    标记物 类型 标记数量 周期
    0.5-1mg 1-2mg
    2-5mg
    HRP 1200 1600 2500 10天
    AP 2400 3600 6400 10天
    Biotin 生物素 1200 1600 2500 10天
    FITC 荧光 1200 1600 2500 10天
    RBITC 荧光 1200 1600 2500 10天
    Cy系列 荧光 1500 2200 3200 10天
    AFluor 荧光 2400 3600 6400 10天
    PE 荧光 2400 3600 6400 10天
    APC 荧光 2400 3600 6400 10天
    PerCP 荧光 3000 4800 - 10天
    PE-Cy双标 荧光 3000 4800 - 15天
    Co-Au 胶体金 1500 2500 4000 10天

    五、标记物介绍:
    碱性磷酸酶(alkaline phosphatase,ALP或AP)是免疫诊断试剂产品最常用的标记酶之一,优点是稳定性高、灵敏度高。ALP是一种能够将对应底物去磷酸化的酶,即通过水解磷酸单酯将底物分子上的磷酸基团除去,并生成磷酸根离子和自由的羟基,这类底物包括核酸、蛋白、生物碱等。而该脱去磷酸基团的过程被称为去磷酸化或脱磷酸化。碱性磷酸酶是磷酸酶的一种,磷酸酶的作用与激酶的作用正相反,激酶是磷酸化酶,可以利用能量分子,如ATP,将磷酸基团加到对应底物分子上。

    辣根过氧化物酶(Horseradish Peroxidase,HRP)活性高,稳定,分子量小,纯酶容易制备,所以最常用。HRP广泛分布于植物界,辣根中含量高,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,分子量为40,000,等电点为PH3~9,酶催化的最适PH因供氢体不同而稍有差异,但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文ReinheitZahl)表示酶的纯度。高纯度的酶RZ值应在3.0左右(最高可达3.4)。RZ值越小,非酶蛋白就越多。

    FITC和RhodamineB(RBITC)是常见的荧光素,性质较稳定;

    Cy系列荧光染料由于荧光较强,对光猝灭有抵抗力而荧光不宜消退,近年来应用比较广泛;





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